For 6-square deepwell microtiter plates, culture volumes between 25 ml and 35 ml are generally used, either at 225 rpm on a shaker with a 25 mm amplitude (photo and video on the left) or at 180 rpm on a shaker with a 50 mm amplitude (photo and video on the right). Under both conditions oxygen transfer rates are approximately 30-40 mmol O 2 l -1 h -1 (corresponding to k L a values of 150-200 h -1 ).
25 mm shaker amplitude 225 rpm
50 mm shaker amplitude 180 rpm
Hydrodynamics inside wells of a 6-square deepwell microplate
Still from high-speed MP4 video below
Still from high-speed MP4 video below