Hydrodynamics inside wells of a 24-roundwell microplate

For 24-roundwell microtiter plates, culture volumes between 0.5  and 1 ml are used, mostly either at 300 rpm on a shaker with a 25 mm amplitude (video on the left) or at 225 rpm on a shaker with a 50 mm amplitude (video on the right).

Under both conditions (at a culture volume of 0.75 ml), oxygen-transfer rates approximate 30 mmol O2 l-1 h-1 (corresponding to kLa values of 150 h-1).

For the (slowed-down) high-speed videos below we used 24-well plates from Corning, which are widely used for the cultivation of bacteria and cells, also because they are worldwide available with various surface treatements (causing different binding characteristics) and gamma irradiated.


Adviced (default) condtion 1: 25 mm shaker amplitude, 300 rpm (E. coli culture on NB, 30 times slowed down)   
0.5 ml 0.75 ml 1 ml

Adviced (default) condtion 2: 50 mm shaker amplitude, 225 rpm (E. coli culture on NB, 20 times slowed down)   
0.5 ml 0.75 ml 1 ml



Below: video database showing alternative shaking conditions

(using E. coli cultures of 0.5, 0.75 and 1 ml,our advice: only apply any of the below conditions if you have a good reason to deviate from the shaking conditions shown above)


25 mm / 200 rpm

50 mm / 200 rpm


25 mm / 225 rpm

50 mm / 250 rpm


25 mm / 250 rpm

50 mm / 275 rpm


25 mm / 275 rpm

50 mm / 300 rpm


25 mm / 325 rpm


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The best way to contact us is to use the form below. However, you may also contact Wouter Duetz directly by e-mail. This e-mail address can be found on the "about us" page.

Optionally you may provide us with additional details about your project; culture type & duration, throughput and desired culture volumes.

We are happy to assist you. We generally reply within a single business day.