Hydrodynamics inside wells of a 6-square deepwell microplate
For 6-square deepwell microtiter plates, culture volumes between 25 ml and 35 ml are generally used, either at 225 rpm on a shaker with a 25 mm amplitude (top photo and video) or at 180 rpm on a shaker with a 50 mm amplitude (bottom photo and video).
Under both conditions oxygen transfer rates are approximately 30-40 mmol O2 l-1 h-1 (corresponding to k La values of 150-200 h-1).
