The cryo-replicator CR1024 has 24 spring-loaded pins and allows the simultaneous sampling of 24 frozen glycerol stocks (-80 ºC) without thawing the remaining glycerol culture. Since the "mother-cultures" remain frozen, their viability (number of CFU's sampled) is not affected by repeated sampling. Per sampling, less than 0.5 µl is removed from each stock culture so that one master plate can be used for several hundreds of times. The pins are 45 mm long and can also be used for the purpose of transferring 24 colonies simultaneously into a deepwell plate containing liquid growth medium.
Want to transfer a larger amount of your cryo-culture? Reach out for availability of the CR1024b, a custom made option with pins of a 6mm diameter.
Publication on different transferring methods and use of the Enzyscreen replicator:
Huber, R., Palmen, T.G., Ryk, N. et al. Replication methods and tools in high-throughput cultivation processes - recognizing potential variations of growth and product formation by on-line monitoring.BMC Biotechnol 10, 22 (2010). https://doi.org/10.1186/1472-6750-10-22
Contact us about orders, quotes and more information
The best way to contact us is to use the form below. However, you may also contact Wouter Duetz directly by e-mail. This e-mail address can be found on the "about us" page.
Optionally you may provide us with additional details about your project; culture type & duration, throughput and desired culture volumes.
We are happy to assist you. We generally reply within a single business day.
