The gastight box with a clamp inside (CR1601) enables microtiter plate fermentations requiring a specific gas-atmosphere (e.g. a higher CO2 concentration, or anearobic cultivations).
The gastight box can be mounted onto shaking platforms from Kuhner, or - after adding some extra holes in the shaker platform - on all other shaker types, and has been constructed to withstand continuous shaking up to 300 rpm at an amplitude of 25 mm, and 225 rpm at an amplitude of 50 mm. Because of these limitations in orbital shaking conditions, we advise to work either with 24 well MTPs and covers or with square half deepwell MTPs in combination with our universal covers for 96- well MTPs (CR 1596b) The gastight box has a gas inlet and a gas outlet (both via a ball-valve), that is compatible with standard 6 mm push-in connectors. The box is gastight (using an EPDM rubber sealing between the perspex box and the carbon groundplate), but is not resistant to overpressure or underpressure more than 0.1 bar. Therefore, the box can not be made anaerobic using vacuum cycles, but has to be flushed instead. The dimensions (groundplate 220x194 mm, total height including ball valves 220mm) allow the transfer in and out most anearobic chambers (via an airlock: vacuum cycles can be used provided the valves of the box are open, or the perspex box and the alu groundplate are transferred into the anaerobic chamber separately). Continuous flushing during shaking is feasible using PTFE tubing (outside diameter 6 mm).