The graphs below show the dependence of oxygen-transfer rates (OTRs) in cultures in 24-square deepwell  MTPs shaken on a shaker with a 50 mm or 25 mm shaking amplitude, at various shaking frequencies and culture volumes. The kLa values are calculated from the OTRs, assuming a maximal concentration of O₂ in the medium of 0.2 mmol per liter.

The data are based on the following publication :

• Duetz, WA, Witholt, B. (2004). Oxygen transfer by orbital shaking of square vessels and deepwell microtiter plates of various dimensions. Biochemical Engineering Journal 17: 181–185

Most data points in the graphs are interpolations and extrapolations of the data in this publication, taking into account the smaller or larger surface-to-volume ratios, as measured from stills from our collection of slowmotion videos.

The graphs also include information on the maximal culture volume where the culture is still fully mixed ("down-to-the-bottom"). Above this critical culture volume, heavy and large cells (such as CHO- or HEK cells, and yeast cells) may precipitate at the bottom (and lead to cell death).

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Graphs for shakers with a 50 mm shaking diameter (in order of decreasing shaking frequencies (300, 275, 250, and 225 rpm):

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Graphs for shakers with a 25 mm shaking diameter (in order of decreasing shaking frequencies (300, 275, 250, and 225 rpm):