Hydrodynamics inside wells of extra-high 24 square deepwell plates

Extra-high 24 square deepwell plates (well volume: 25 ml) are applied when culture volumes of 2.5 to 3.5 ml (as can be maximally used for regular 24 square deepwell plates) do not suffice.

With extra-high 24 square deepwell plates, culture volumes up till 8 ml can be used, preferably at 300 rpm on a shaker with a 50 mm amplitude.

Oxygen-transfer rates between 40 and 60 mmol O2 l-1 h-1  (corresponding to kLa values of 200-300 h-1) are readily attainable.

For the (slowed-down) high-speed videos below we used hitplates from HJ- Bioanalytik

 

Adviced (default) condtion 1: 

25 mm shaker amplitude, 375 rpm

    5 ml, 6 ml, 7 ml and 8ml           
(E. coli culture on NB, 20 times slowed down)

 

Adviced (default) condtion 2: 

50 mm shaker amplitude, 300 rpm

5 ml, 6 ml, 7 ml and 8ml           
(E. coli culture on NB, 20 times slowed down)

 

 


Below: video database showing alternative shaking conditions (using E. coli cultures of 5 , 6, 7, or 8 ml)

 

25 mm / 300 rpm

50 mm / 225 rpm

25 mm / 325 rpm

50 mm / 250 rpm

25 mm / 350 rpm

50 mm / 275 rpm

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Optionally you may provide us with additional details about your project; culture type & duration, throughput and desired culture volumes.

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