Hydrodynamics inside wells of a 6-square deepwell microplate

For 6-square deepwell microtiter plates, culture volumes between 25 ml and 35 ml are generally used, either at 225 rpm on a shaker with a 25 mm amplitude (top photo and video) or at 180 rpm on a shaker with a 50 mm amplitude (bottom photo and video).

Under both conditions oxygen transfer rates  are approximately 30-40 mmol O2 l-1 h-1 (corresponding to k La values of 150-200 h-1).

Still from high-speed MP4 video below, 25 mm shaker amplitude, 225 rpm, 25ml - 30ml - 35ml from left to right

 


 

Still from high-speed MP4 video below, 50 mm shaker amplitude, 180 rpm, 25ml - 30ml - 35ml from left to right

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The best way to contact us is to use the form below. However, you may also contact Wouter Duetz directly by e-mail. This e-mail address can be found on the "about us" page.

Optionally you may provide us with additional details about your project; culture type & duration, throughput and desired culture volumes.

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