“System Duetz”A mature, easy-to-use, and cost-effective technology platform for the high-quality cultivation in microplates of large numbers of microbial strains, clonal libraries, mutant banks, CHO-cells, etc.In comparison to Erlenmeyer flasks, the same quality in terms of mixing, oxygen-transfer rates and reproducibility is readily achieved, provided the right orbital shaking conditions are applied (see our pages on the hydrodynamics inside the wells during orbital shaking).So-called “sandwich covers” and clamp systems are available for:•6-round and square well plates (3-35 ml cultures)•24-square deepwell and extra-high deepwell plates (2.5 - 8 ml cultures)•24-round lowwell plates (0.75 - 1 ml cultures)•96-square deepwell and half deepwell plates (0.3 - 0.75 ml cultures)•96-round lowwell plates (0.1 - 0.2 ml cultures)The system was originally developped and validated at the Institute of Biotechnology of the ETH Zurich: read the key publication from the year 2000 in Applied and Environmental Microbiology (Duetz et al. 2000, pdf-file [146 kb]).
Application areas:- High-throughput screening for new enzyme activities or formation of secondary metabolites (heterogeneous culture collections)- Cultivation of CHO cells and other animal cell lines (see dedicated page on CHO-cells)- Screening and distribution of clone libraries in E. coli or yeasts (see page on bacteria)- High-throughput screening for high-activity or high-productivity mutants (see page on bacteria)- Comparative studies e.g. clinical isolates- Growth medium optimization studies
Main components:Sandwich covers convert all wells of microtiter plates into individual "mini-reactors" suitable for high-frequency orbital shaking, at high oxygen transfer rates, and without well-to-well variations.Cover clamps ensure that the deepwell plate and its sandwich cover are clamped together tightly, thereby preventing well-to-well contamination.The cryo-replicator: a precision spring-loaded device for the simultaneous and reproducible sampling of 96 frozen glycerol stocks. With the CRYO-REPLICATOR culture stocks remain viable for years because they can remain frozen.The cryo-replicator press is a guiding system ensuring accuracy during the replication of sets of strains
General procedure used to generate active suspended cultures, starting from a master-plate with 96 individual strains stored at - 80 ºC in the presence of 15% (v/v) glycerol
Our cryo-replicator CR1000
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