For 6-square deepwell microtiter plates, culture volumes between 25 ml and 35 ml are generally used, either at 225 rpm on a shaker with a 25 mm amplitude (photo and video on the left) or at 180 rpm on a shaker with a 50 mm amplitude (photo and video on the right).Under both conditions oxygen transfer rates are approximately 30-40 mmol O2 l-1 h-1 (corresponding to k La values of 150-200 h-1).
•25 mm shaker amplitude•225 rpm
•50 mm shaker amplitude•180 rpm
Hydrodynamics inside wells of a 6-square deepwell microplate