For 6-square deepwell microtiter plates, culture volumes between 25 ml and 35 ml are generally used, either at 225 rpm on a shaker with a 25 mm
amplitude (photo and video on the left) or at 180 rpm on a shaker with a 50 mm amplitude (photo and video on the right).
Under both conditions oxygen transfer rates are approximately 30-40 mmol O2 l-1 h-1 (corresponding to k La values of 150-200 h-1).
•
25 mm shaker
amplitude
•
225 rpm
•
50 mm shaker
amplitude
•
180 rpm
Hydrodynamics inside wells of a 6-square deepwell microplate
Still from high-speed MP4 video below
Still from high-speed MP4 video below
