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Extra-high 24 square deepwell plates (well volume: 25 ml) are applied when culture volumes of 2.5 to 3.5 ml (as can be maximally used for regular 24 square deepwell plates) do not suffice. With extra-high 24 square deepwell plates, culture volumes up till 8 ml can be used, preferably at 300 rpm on a shaker with a 50 mm amplitude Oxygen-transfer rates between 40 and 60 mmol O2 l-1 h-1  (corresponding to kLa values of 200-300 h-1) are readily attainable. For the (slowed-down) high-speed videos below we used hitplates from HJ- Bioanalytik
Adviced (default) condtion 1: 25 mm shaker amplitude, 375 rpm (E. coli culture on NB, 20 times slowed down)     5 ml       6 ml      7 ml       8ml
Hydrodynamics inside wells of  extra-high 24 square deepwell plates
Adviced (default) condtion 2: 50 mm shaker amplitude, 300 rpm (E. coli culture on NB, 20 times slowed down)         5 ml       6 ml      7 ml       8ml
Below: video database showing alternative shaking conditions (using E. coli cultures of 5 , 6, 7, or 8 ml),
25 mm / 300 rpm
25 mm / 325 rpm
25 mm / 350 rpm
50 mm / 225 rpm
50 mm / 250 rpm
50 mm / 275 rpm